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KD/KO Validated

HBS1L Polyclonal antibody

Name: HBS1L Polyclonal antibody
Brand: Proteintech
SKU: 10359-1-AP

HBS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10359-1-AP

Synonyms

HSPC276, HBS1, HBS 1, ERFS, EF 1a

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Jurkat using 10359-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10359-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10359-1-AP
WB result of HBS1L antibody (10359-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.
WB analysis of Jurkat using 10359-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10359-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 10359-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10359-1-AP
IP result of anti-HBS1L (IP:10359-1-AP, 4ug; Detection:10359-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human pancreas cancer using 10359-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10359-1-AP (HBS1L antibody) at dilution of 1:50 (under 25x lens).
IF Staining of HepG2 using 10359-1-AP
Immunofluorescent analysis of HepG2 cells, using HBS1L antibody 10359-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 10359-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, A2780 cells, HEK-293 cells, mouse brain tissue, SKOV-3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IF	See 1 publications below

Product Information

10359-1-AP targets HBS1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HBS1L fusion protein Ag0388 相同性解析による交差性が予測される生物種
Full Name	HBS1-like (S. cerevisiae)
Calculated molecular weight	75 kDa
Observed molecular weight	75 kDa
GenBank accession number	BC001465
Gene symbol	HBS1L
Gene ID (NCBI)	10767
RRID	AB_2114730
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

Protocols

Product Specific Protocols
WB protocol for HBS1L antibody 10359-1-AP	Download protocol
IHC protocol for HBS1L antibody 10359-1-AP	Download protocol
IF protocol for HBS1L antibody 10359-1-AP	Download protocol
IP protocol for HBS1L antibody 10359-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes. Authors - Michael K Slevin View Article
mouse	WB	Elife Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology. Authors - Markus Terrey View Article
human	WB	Biochem J Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation. Authors - John Rp Knight View Article
human	WB	J Biol Chem RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism. Authors - Hussein H Aly KD Validated View Article
mouse	WB	PLoS Genet Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation. Authors - Amy E O'Connell KO Validated View Article
mouse	WB	Immunity Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity Authors - Xiurong Wu KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HBS1L Polyclonal antibody

HBS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 10359-1-AP

WB analysis using 10359-1-AP

WB analysis of HEK-293 using 10359-1-AP

WB analysis of Jurkat using 10359-1-AP

WB analysis of mouse brain using 10359-1-AP

WB analysis of A2780 using 10359-1-AP

IP experiment of mouse brain using 10359-1-AP

IHC staining of human pancreas cancer using 10359-1-AP

IF Staining of HepG2 using 10359-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.

Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.

Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.

RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism.

Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.

Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity