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  • KD/KO Validated

HBS1L Polyclonal antibody

HBS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10359-1-AP

Synonyms

HSPC276, HBS1, HBS 1, ERFS, EF 1a


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Tested Applications

Positive WB detected inJurkat cells, A2780 cells, HEK-293 cells, mouse brain tissue, SKOV-3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10359-1-AP targets HBS1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HBS1L fusion protein Ag0388 相同性解析による交差性が予測される生物種
Full Name HBS1-like (S. cerevisiae)
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC001465
Gene symbol HBS1L
Gene ID (NCBI) 10767
RRIDAB_2114730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

Protocols

Product Specific Protocols
WB protocol for HBS1L antibody 10359-1-APDownload protocol
IHC protocol for HBS1L antibody 10359-1-APDownload protocol
IF protocol for HBS1L antibody 10359-1-APDownload protocol
IP protocol for HBS1L antibody 10359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.

Authors - Michael K Slevin
mouseWB

Elife

Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.

Authors - Markus Terrey
humanWB

Biochem J

Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.

Authors - John Rp Knight
humanWB

J Biol Chem

RNA exosome complex regulates stability of Hepatitis B Virus's X-mRNA transcript in a Non-Stop mediated (NSD) RNA quality control mechanism.

Authors - Hussein H Aly
  • KD Validated
mouseWB

PLoS Genet

Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.

Authors - Amy E O'Connell
  • KO Validated
mouseWB

Immunity

Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity

Authors - Xiurong Wu
  • KO Validated