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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HBS1L Recombinant antibody (82795-2-RR)

    WB analysis using 82795-2-RR (same clone as 82795-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82795-2-RR (HBS1L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82795-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HBS1L Recombinant antibody (82795-2-RR)

    WB analysis of HEK-293 using 82795-2-RR (same clone as 82795-2-PBS)

    WB result of HBS1L antibody (82795-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 82795-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82795-2-PBS

    Affinity and Kinetic Characterization of 82795-2-PBS

    Biolayer interferometry (BLl) kinetic assays of 82795-2-RR against Human HBS1L were performed. The affinity constant is 0.931 nM.

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HBS1L Recombinant antibody, PBS Only

HBS1L Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 82795-2-PBS
Clone No.2L24

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82795-2-PBS targets HBS1L in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HBS1L fusion protein Ag0388 相同性解析による交差性が予測される生物種
Full Name HBS1-like (S. cerevisiae)
Calculated molecular weight 75 kDa
Observed molecular weight78 kDa
GenBank accession numberBC001465
Gene Symbol HBS1L
Gene ID (NCBI) 10767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y450
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.