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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using HBZ Monoclonal antibody (67878-1-Ig)

    WB analysis of K-562 using 67878-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67878-1-Ig (HBZ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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HBZ Monoclonal antibody

HBZ Monoclonal Antibody for WB, ELISA
Cat No. 67878-1-Ig
Clone No.1F9C2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

HBAZ, HBZ, HBZ2, Hemoglobin subunit zeta, Hemoglobin zeta chain, hemoglobin, zeta, Zeta globin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67878-1-Ig targets HBZ in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HBZ fusion protein Ag11061 相同性解析による交差性が予測される生物種
Full Name hemoglobin, zeta
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight14-16 kDa
GenBank accession numberBC027892
Gene Symbol HBZ
Gene ID (NCBI) 3050
RRIDAB_2918635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HBZ, also named as Zeta-globin and HBAZ, belongs to the globin family. HBZ is an alpha-type chain of mammalian embryonic hemoglobin, synthesized primarily in the yolk sac.

Protocols

Product Specific Protocols
WB protocol for HBZ antibody 67878-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols