HCCS Polyclonal antibody

Name: HCCS Polyclonal antibody
Brand: Proteintech
SKU: 15118-1-AP

HCCS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15118-1-AP

Synonyms

Holocytochrome c-type synthase, Holocytochrome c type synthase, EC:4.4.1.17, Cytochrome c-type heme lyase, Cytochrome c type heme lyase

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of human heart using 15118-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15118-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15118-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 15118-1-AP
IP result of anti-HCCS (IP:15118-1-AP, 4ug; Detection:15118-1-AP 1:500) with MCF-7 cells lysate 3200ug.
IHC staining of human breast cancer using 15118-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15118-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15118-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15118-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15118-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15118-1-AP (HCCS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15118-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human heart tissue, HeLa cells, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

15118-1-AP targets HCCS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HCCS fusion protein Ag7189 相同性解析による交差性が予測される生物種
Full Name	holocytochrome c synthase (cytochrome c heme-lyase)
Calculated molecular weight	31 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC001691
Gene symbol	HCCS
Gene ID (NCBI)	3052
RRID	AB_2114848
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HCCS(Cytochrome c-type heme lyase) links covalently the heme group to the apoprotein of cytochrome C.The covalent attachment of heme by CCHL in the IMS is crucial for translocation of the precursor of cytochrome c and its trapping in the IMS(PMID:15359280 ).This protein has a calculated molecular mass of about 31 KDa.

Protocols

Product Specific Protocols
WB protocol for HCCS antibody 15118-1-AP	Download protocol
IHC protocol for HCCS antibody 15118-1-AP	Download protocol
IF protocol for HCCS antibody 15118-1-AP	Download protocol
IP protocol for HCCS antibody 15118-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. Authors - Alexandre Janer View Article
human	WB	Chem Pharm Bull (Tokyo) The Novel Synthetic Triterpene Methyl 3β-O-[4-(2-Aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate Inhibits Breast Tumor Cell Growth in Vitro and in Vivo. Authors - Bin Feng View Article
human,mouse	WB	Nat Cell Biol Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space Authors - Elias Adriaenssens View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HCCS Polyclonal antibody

HCCS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of human heart using 15118-1-AP

WB analysis of MCF-7 using 15118-1-AP

WB analysis of HeLa using 15118-1-AP

IP experiment of MCF-7 using 15118-1-AP

IHC staining of human breast cancer using 15118-1-AP

IHC staining of human breast cancer using 15118-1-AP

IHC staining of human breast cancer using 15118-1-AP

IHC staining of human breast cancer using 15118-1-AP

IF Staining of HeLa using 15118-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.

The Novel Synthetic Triterpene Methyl 3β-O-[4-(2-Aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate Inhibits Breast Tumor Cell Growth in Vitro and in Vivo.

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space