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HCFC1 Polyclonal antibody

HCFC1 Polyclonal Antibody for WB, ELISA
Cat No. 19358-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

19358-1-AP targets HCFC1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HCFC1 fusion protein Ag6798 相同性解析による交差性が予測される生物種
Full Name host cell factor C1 (VP16-accessory protein)
Calculated molecular weight 209 kDa
Observed molecular weight 230 kDa, 300 kDa
GenBank accession numberBC063435
Gene Symbol HCFC1
Gene ID (NCBI) 3054
RRIDAB_10860105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51610
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Host cell factor 1 (HCFC1), also known as HCF-1, is a transcriptional co-regulator with a proteolytic cleavage at the PRO repeats that could be cleaved by O-GlcNAc transferase (OGT) to produce a heterodimeric complex of HCFC1N and HCFC1C subunits (PMID: 23539139). HCFC1 was first identified in herpes simplex virus transcription while it lacks a DNA binding domain. HCFC1 is not a traditional transcription factor, it interacts with multiple protein including transcription factors to regulate many biological processes, such as cell proliferation, migration, cell death and mitochondrial biogenesis (PMID: 25281006). Mutation in HCFC1 was related to an X-linked recessive disease ---cblX with a complex clinical manifestation involved in error of metabolism,  neurocognitive impairment and  craniofacial abnormalities (PMID: 24011988; PMID: 25281006).

Protocols

Product Specific Protocols
WB protocol for HCFC1 antibody 19358-1-APDownload protocol
IHC protocol for HCFC1 antibody 19358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratIF

Microbiol Immunol

Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation-dependent.

Authors - Erina Minato
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