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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HCLS1 Polyclonal antibody

HCLS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25003-1-AP

Synonyms

p75, LckBP1, HS1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HCLS1 Polyclonal antibody (25003-1-AP)

    WB analysis of Jurkat using 25003-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25003-1-AP (HCLS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HCLS1 Polyclonal antibody (25003-1-AP)

    IHC staining of human tonsillitis using 25003-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25003-1-AP (HCLS1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HCLS1 Polyclonal antibody (25003-1-AP)

    IHC staining of human tonsillitis using 25003-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25003-1-AP (HCLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25003-1-AP targets HCLS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HCLS1 fusion protein Ag8645 相同性解析による交差性が予測される生物種
Full Name hematopoietic cell-specific Lyn substrate 1
Calculated molecular weight 486 aa, 54 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC016758
Gene symbol HCLS1
Gene ID (NCBI) 3059
RRIDAB_2879842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hematopoietic cell-specific Lyn substrate 1 (HS1, HCLS1, LckBP1 and p75) is a 75-kDa intracellular protein expressed in cells of lymphohemopoietic origin. It was originally identified in B-lymphocytes as a major substrate of B-cell receptor (BCR)-induced phosphorylation after antigen (Ag) stimulation. It's a substrate of the antigen receptor-coupled tyrosine kinase. HCLS1 plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells.

Protocols

Product Specific Protocols
WB protocol for HCLS1 antibody 25003-1-APDownload protocol
IHC protocol for HCLS1 antibody 25003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.

Authors - Jinguo Zhang
View Article
canineWB

Vaccines (Basel)

Transcriptional Analysis of lncRNA and Target Genes Induced by Influenza A Virus Infection in MDCK Cells

Authors - Geng Liu
View Article