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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HCLS1 Recombinant antibody (85114-1-RR)

    WB analysis using 85114-1-RR (same clone as 85114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat thymus tissue using HCLS1 Recombinant antibody (85114-1-RR)

    WB analysis of rat thymus using 85114-1-RR (same clone as 85114-1-PBS)

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using HCLS1 Recombinant antibody (85114-1-RR)

    WB analysis of mouse thymus using 85114-1-RR (same clone as 85114-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01860-1

    Sandwich ELISA standard curve of MP01860-1

    Sandwich ELISA standard curve of MP01860-1, Human HCLS1 Recombinant Matched Antibody Pair - PBS only. 85114-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8645. 85114-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01860-1

    Sample test of MP01860-1

    The mean HCLS1 concentration was determined to be 541.92 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load, 1,269.82 ng/mL in Daudi cell extract based on a 3.60 mg/mL extract load and 889.93 ng/mL in Jurkat cell extract based on a 3.10 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HEL cells using HCLS1 Recombinant antibody (85114-1-RR)

    IF Staining of HEL using 85114-1-RR (same clone as 85114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using HCLS1 Recombinant antibody (85114-1-RR)

    IF Staining of Jurkat using 85114-1-RR (same clone as 85114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01860-1

    Cytometric bead array standard curve of MP01860-1

    Cytometric bead array standard curve of MP01860-1, HCLS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85114-1-PBS. Detection antibody: 85114-2-PBS. Standard: Ag8645. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01860-2

    Cytometric bead array standard curve of MP01860-2

    Cytometric bead array standard curve of MP01860-2, HCLS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85114-1-PBS. Detection antibody: 85114-3-PBS. Standard: Ag8645. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85114-1-RR

    Affinity and Kinetic Characterization of 85114-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85114-1-RR against Human HCLS1 were performed. The affinity constant is 4.71 nM.

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HCLS1 Recombinant antibody, PBS Only (Capture)

HCLS1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85114-1-PBS
Clone No.242825E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242825E4, CTTNL, Hematopoietic cell-specific LYN substrate 1, Hematopoietic lineage cell-specific protein, HS1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85114-1-PBS targets HCLS1 as part of a matched antibody pair:

MP01860-1: 85114-1-PBS capture and 85114-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01860-2: 85114-1-PBS capture and 85114-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HCLS1 fusion protein Ag8645 相同性解析による交差性が予測される生物種
Full Name hematopoietic cell-specific Lyn substrate 1
Calculated molecular weight 486 aa, 54 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC016758
Gene Symbol HCLS1
Gene ID (NCBI) 3059
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14317
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Hematopoietic cell-specific Lyn substrate 1 (HS1, HCLS1, LckBP1 and p75) is a 75-kDa intracellular protein expressed in cells of lymphohemopoietic origin. It was originally identified in B-lymphocytes as a major substrate of B-cell receptor (BCR)-induced phosphorylation after antigen (Ag) stimulation. It's a substrate of the antigen receptor-coupled tyrosine kinase. HCLS1 plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells.