Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30946-1-AP targets HCN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HCN2 fusion protein Ag34038 相同性解析による交差性が予測される生物種 |
| Full Name | hyperpolarization activated cyclic nucleotide-gated potassium channel 2 |
| Calculated molecular weight | 97 kDa |
| Observed molecular weight | 60 kDa, 90 kDa, 120 kDa |
| GenBank accession number | NM_001194 |
| Gene Symbol | HCN2 |
| Gene ID (NCBI) | 610 |
| RRID | AB_3086427 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL51 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HCN2 antibody 30946-1-AP | Download protocol |
| IHC protocol for HCN2 antibody 30946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther HCN channels in the lateral habenula regulate pain and comorbid depressive-like behaviors in mice |