☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

HCRTR1 Polyclonal antibody

HCRTR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18370-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Orexin receptor 1, Ox 1 R, Orexin/Hypocretin receptor type 1, Orexin receptor type 1, Hypocretin receptor type 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18370-1-AP targets HCRTR1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HCRTR1 fusion protein Ag12269 相同性解析による交差性が予測される生物種
Full Name hypocretin (orexin) receptor 1
Calculated molecular weight 425 aa, 48 kDa
Observed molecular weight 48-54 kDa
GenBank accession numberBC074796
Gene Symbol HCRTR1
Gene ID (NCBI) 3061
RRIDAB_10858633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).

Protocols

Product Specific Protocols
WB protocol for HCRTR1 antibody 18370-1-APDownload protocol
IHC protocol for HCRTR1 antibody 18370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Front Pharmacol

The Ciji-Hua'ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma.

Authors - Shengyan Xi
mouseWB

Brain Res Bull

Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism.

Authors - Pedro P M Scariot
mouseWB

Drug Des Devel Ther

Orexin-A Reverse Bone Mass Loss Induced by Chronic Intermittent Hypoxia Through OX1R-Nrf2/HIF-1α Pathway.

Authors - Hong Gu
ratWB

Evid Based Complement Alternat Med

Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A.

Authors - Liang-Hui Zhan
humanWB

Biochim Biophys Acta Mol Basis Dis

Orexin-A/OX1R is involved in regulation of autophagy to promote cortisol secretion in adrenocortical cell

Authors - Xin Guo
mouseIF

Nat Biotechnol

Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells

Authors - Yunlong Tao
Loading...