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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC1 Recombinant antibody

HDAC1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240614A3

Cat no : 83624-1-RR

Synonyms

histone deacetylase 1, HD1, GON 10, EC:3.5.1.98, EC:3.5.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC1 Recombinant antibody (83624-1-RR)

    WB analysis using 83624-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83624-1-RR (HDAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    IF Staining of HeLa using 83624-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    IF Staining of HeLa using 83624-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    FC experiment of HeLa using 83624-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HDAC1 Recombinant antibody (83624-1-RR, Clone:240614A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83624-1-RR

    Affinity and Kinetic Characterization of 83624-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83624-1-RR against Human HDAC1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, HeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83624-1-RR is HDAC1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HDAC1 fusion protein Ag0256 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 1
Calculated molecular weight 55 kDa
Observed molecular weight 58-66 kDa
GenBank accession numberBC000301
Gene symbol HDAC1
Gene ID (NCBI) 3065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus.

Protocols

Product Specific Protocols
WB protocol for HDAC1 antibody 83624-1-RRDownload protocol
IF protocol for HDAC1 antibody 83624-1-RRDownload protocol
FC protocol for HDAC1 antibody 83624-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols