HDAC1 Recombinant antibody
HDAC1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240614A3
Cat no : 83624-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, Jurkat cells, K-562 cells, HeLa cells, NIH/3T3 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83624-1-RR is HDAC1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | HDAC1 fusion protein Ag0256 相同性解析による交差性が予測される生物種 |
Full Name | histone deacetylase 1 |
Calculated molecular weight | 55 kDa |
Observed molecular weight | 58-66 kDa |
GenBank accession number | BC000301 |
Gene symbol | HDAC1 |
Gene ID (NCBI) | 3065 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus.
Protocols
Product Specific Protocols | |
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WB protocol for HDAC1 antibody 83624-1-RR | Download protocol |
IF protocol for HDAC1 antibody 83624-1-RR | Download protocol |
FC protocol for HDAC1 antibody 83624-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |