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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC1-specific Polyclonal antibody

HDAC1-specific Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16160-1-AP

Synonyms

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using HDAC1-specific Polyclonal antibody (16160-1-AP)

    WB analysis of mouse testis using 16160-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using HDAC1-specific Polyclonal antibody (16160-1-AP)

    WB analysis of mouse testis using 16160-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using HDAC1-specific Polyclonal antibody (16160-1-AP)

    WB analysis of mouse thymus using 16160-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using HDAC1-specific Polyclonal antibody (16160-1-AP)

    WB analysis of mouse brain using 16160-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using HDAC1-specific Polyclonal antibody (16160-1-AP)

    IP experiment of mouse testis using 16160-1-AP

    IP result of anti-HDAC1-specific (IP:16160-1-AP, 4ug; Detection:16160-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HDAC1-specific Polyclonal antibody (16160-1-AP)

    IF Staining of HepG2 using 16160-1-AP

    Immunofluorescent analysis of HepG2 cells using 16160-1-AP (HDAC1-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inmouse testis tissue, mouse brain tissue, mouse thymus tissue
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16160-1-AP targets HDAC1-specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 1
Calculated molecular weight 55 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_004964
Gene symbol HDAC1
Gene ID (NCBI) 3065
RRIDAB_2118060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.

Protocols

Product Specific Protocols
WB protocol for HDAC1-specific antibody 16160-1-APDownload protocol
IF protocol for HDAC1-specific antibody 16160-1-APDownload protocol
IP protocol for HDAC1-specific antibody 16160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols