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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC10 Recombinant antibody

HDAC10 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240877E1

Cat no : 83788-4-RR

Synonyms

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HDAC10 Recombinant antibody (83788-4-RR)

    WB analysis of Jurkat using 83788-4-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 83788-4-RR (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83788-4-RR

    Affinity and Kinetic Characterization of 83788-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83788-4-RR against Human HDAC10 were performed. The affinity constant is 0.476 nM.

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83788-4-RR is HDAC10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HDAC10 fusion protein Ag18851 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 10
Calculated molecular weight 669 aa, 71 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC125083
Gene symbol HDAC10
Gene ID (NCBI) 83933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Protocols

Product Specific Protocols
WB protocol for HDAC10 antibody 83788-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols