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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HDAC2-specific Polyclonal antibody (16152-1-AP)

    WB analysis of HepG2 using 16152-1-AP (same clone as 16152-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using HDAC2-specific Polyclonal antibody (16152-1-AP)

    WB analysis of A431 using 16152-1-AP (same clone as 16152-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    WB analysis of human liver using 16152-1-AP (same clone as 16152-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HDAC2-specific Polyclonal antibody (16152-1-AP)

    WB analysis of HeLa using 16152-1-AP (same clone as 16152-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IP experiment of A431 using 16152-1-AP (same clone as 16152-1-PBS)

    IP result of anti-HDAC2-specific (IP:16152-1-AP, 4ug; Detection:16152-1-AP 1:500) with A431 cells lysate 2400ug. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human testis using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human testis using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human skin using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human skin using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human brain using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human brain using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human liver using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human liver using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human spleen using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human spleen using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human ovary using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human heart using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human heart using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human kidney using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human kidney using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IHC staining of human ovary using 16152-1-AP (same clone as 16152-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC2-specific Polyclonal antibody (16152-1-AP)

    IF Staining of HeLa using 16152-1-AP (same clone as 16152-1-PBS)

    Immunofluorescent analysis of HeLa cells using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

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HDAC2-specific Polyclonal antibody, PBS Only

HDAC2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16152-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16152-1-PBS targets HDAC2-specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 2
Calculated molecular weight 458 aa, 52 kDa; 488 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_001527
Gene Symbol HDAC2
Gene ID (NCBI) 3066
RRIDAB_2118514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92769
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.