Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 16152-1-AP (same clone as 16152-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 16152-1-AP (same clone as 16152-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 16152-1-AP (same clone as 16152-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16152-1-AP (same clone as 16152-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IP experiment of A431 using 16152-1-AP (same clone as 16152-1-PBS)
IP result of anti-HDAC2-specific (IP:16152-1-AP, 4ug; Detection:16152-1-AP 1:500) with A431 cells lysate 2400ug. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 16152-1-AP (same clone as 16152-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 16152-1-AP (same clone as 16152-1-PBS)
Immunofluorescent analysis of HeLa cells using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 16152-1-PBS in a different storage buffer formulation.

HDAC2-specific Polyclonal antibody, PBS Only

HDAC2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16152-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16152-1-PBS targets HDAC2-specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	histone deacetylase 2
Calculated molecular weight	458 aa, 52 kDa; 488 aa, 55 kDa
Observed molecular weight	55 kDa
GenBank accession number	NM_001527
Gene Symbol	HDAC2
Gene ID (NCBI)	3066
RRID	AB_2118514
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92769
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 16152-1-AP (same clone as 16152-1-PBS)

WB analysis of A431 using 16152-1-AP (same clone as 16152-1-PBS)

WB analysis of human liver using 16152-1-AP (same clone as 16152-1-PBS)

WB analysis of HeLa using 16152-1-AP (same clone as 16152-1-PBS)

IP experiment of A431 using 16152-1-AP (same clone as 16152-1-PBS)

IHC staining of human testis using 16152-1-AP (same clone as 16152-1-PBS)

IHC staining of human testis using 16152-1-AP (same clone as 16152-1-PBS)

IHC staining of human skin using 16152-1-AP (same clone as 16152-1-PBS)

IHC staining of human skin using 16152-1-AP (same clone as 16152-1-PBS)

IHC staining of human brain using 16152-1-AP (same clone as 16152-1-PBS)

IHC staining of human ovary using 16152-1-AP (same clone as 16152-1-PBS)