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  • Featured Product
  • KD/KO Validated

HDAC4 Monoclonal antibody, PBS Only

HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2G12

Cat no : 66838-1-PBS

Synonyms

KIAA0288, HD4, EC:3.5.1.98, 1D2G12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC4 Monoclonal antibody (66838-1-Ig)

    WB analysis using 66838-1-Ig (same clone as 66838-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66838-1-Ig (HDAC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDAC4 Monoclonal antibody (66838-1-Ig)

    IHC staining of human breast cancer using 66838-1-Ig (same clone as 66838-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDAC4 Monoclonal antibody (66838-1-Ig)

    IHC staining of human breast cancer using 66838-1-Ig (same clone as 66838-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using HDAC4 Monoclonal antibody (66838-1-Ig)

    IF Staining of mouse liver using 66838-1-Ig (same clone as 66838-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using HDAC4 Monoclonal antibody (66838-1-Ig)

    IF Staining of C2C12 using 66838-1-Ig (same clone as 66838-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66838-1-PBS targets HDAC4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HDAC4 fusion protein Ag11345 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 4
Calculated molecular weight 972 aa, 106 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC039904
Gene symbol HDAC4
Gene ID (NCBI) 9759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.