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  • KD/KO Validated

HDAC4-specific Polyclonal antibody

HDAC4-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 16165-1-AP

Synonyms

HDAC4, KIAA0288, HD4, EC:3.5.1.98


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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16165-1-AP targets HDAC4-specific in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 4
Calculated molecular weight 119 kDa
Observed molecular weight 119-140 kDa
GenBank accession numberBC039904
Gene symbol HDAC4
Gene ID (NCBI) 9759
RRIDAB_2279763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC4.

Protocols

Product Specific Protocols
WB protocol for HDAC4-specific antibody 16165-1-APDownload protocol
IHC protocol for HDAC4-specific antibody 16165-1-APDownload protocol
IF protocol for HDAC4-specific antibody 16165-1-APDownload protocol
IP protocol for HDAC4-specific antibody 16165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi
humanWB

Hepatology

The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma.

Authors - Yuan Ji-hang JH
  • KD Validated
mouseWB

J Orthop Translat

Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta

Authors - Bin Sun
mouseWB

Biol Trace Elem Res

Exercise Alleviates Fluoride-Induced Learning and Memory Impairment in Mice: Role of miR-206-3p and PREG

Authors - Lei Chai