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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC5 Polyclonal antibody

HDAC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29342-1-AP

Synonyms

Antigen NY CO 9, FLJ90614, HD5, HDAC, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC5 Polyclonal antibody (29342-1-AP)

    WB analysis using 29342-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29342-1-AP (HDAC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HDAC5 Polyclonal antibody (29342-1-AP)

    IHC staining of mouse brain using 29342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29342-1-AP (HDAC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HDAC5 Polyclonal antibody (29342-1-AP)

    IF Staining of HepG2 using 29342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC5 antibody (29342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29342-1-AP targets HDAC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC5 fusion protein Ag29122 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 5
Calculated molecular weight 122 kDa
Observed molecular weight120-140 kDa
GenBank accession numberNM_005474
Gene symbol HDAC5
Gene ID (NCBI) 10014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.

Protocols

Product Specific Protocols
WB protocol for HDAC5 antibody 29342-1-APDownload protocol
IHC protocol for HDAC5 antibody 29342-1-APDownload protocol
IF protocol for HDAC5 antibody 29342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols