Validation Data Gallery
Tested Applications
| Positive WB detected in | Saos-2 cells, COLO 320 cells, HeLa cells, T-47D cells, SK-BR-3 cells, A2780 cells, HepG2 cells, HSC-T6 cells, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68437-1-Ig targets HDAC5 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HDAC5 fusion protein Ag30557 相同性解析による交差性が予測される生物種 |
| Full Name | histone deacetylase 5 |
| Calculated molecular weight | 122 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_005474 |
| Gene Symbol | HDAC5 |
| Gene ID (NCBI) | 10014 |
| RRID | AB_3085150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UQL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HDAC5 antibody 68437-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |