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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC6 Monoclonal antibody, PBS Only

HDAC6 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7C3

Cat no : 67250-1-PBS

Synonyms

FLJ16239, HD6, HDAC, HDAC6, histone deacetylase 6, JM21, KIAA0901

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC6 Monoclonal antibody (67250-1-Ig)

    WB analysis using 67250-1-Ig (same clone as 67250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67250-1-Ig (HDAC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HDAC6 Monoclonal antibody (67250-1-Ig)

    WB analysis of HeLa using 67250-1-Ig (same clone as 67250-1-PBS)

    WB result of HDAC6 antibody (67250-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC6 transfected HeLa cells. This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDAC6 Monoclonal antibody (67250-1-Ig)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDAC6 Monoclonal antibody (67250-1-Ig)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HDAC6 Monoclonal antibody (67250-1-Ig)

    IHC staining of human breast cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HDAC6 Monoclonal antibody (67250-1-Ig)

    IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HDAC6 Monoclonal antibody (67250-1-Ig)

    IHC staining of human lung cancer using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67250-1-Ig (HDAC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using HDAC6 Monoclonal antibody (67250-1-Ig)

    IF Staining of SH-SY5Y using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HDAC6 Monoclonal antibody (67250-1-Ig)

    IF Staining of HepG2 using 67250-1-Ig (same clone as 67250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDAC6 antibody (67250-1-Ig, Clone: 1C7C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67250-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67250-1-PBS targets HDAC6 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HDAC6 fusion protein Ag28585 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 6
Calculated molecular weight 1063 aa, 114 kDa, 131 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC013737
Gene symbol HDAC6
Gene ID (NCBI) 10013
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.