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HDAC8 Polyclonal antibody

HDAC8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17548-1-AP

Synonyms

Protein decrotonylase HDAC8, Protein deacetylase HDAC8, HDAC 8, EC:3.5.1.98, EC:3.5.1.-


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Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inRaji cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17548-1-AP targets HDAC8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC8 fusion protein Ag11696 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 8
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC050433
Gene symbol HDAC8
Gene ID (NCBI) 55869
RRIDAB_2116926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

Protocols

Product Specific Protocols
WB protocol for HDAC8 antibody 17548-1-APDownload protocol
IF protocol for HDAC8 antibody 17548-1-APDownload protocol
IP protocol for HDAC8 antibody 17548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
ratWB

Cell Rep

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.

Authors - Tian Wang
mouse, ratWB

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang
humanWB

Oncotarget

Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair.

Authors - Pei Chen
humanWB

Oncogenesis

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Authors - Wei Zhan
humanWB

Molecules

Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway

Authors - Xintian Lan