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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC9 Polyclonal antibody

HDAC9 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28334-1-AP

Synonyms

DKFZp779K1053, HD7, HD7b, HD9, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC9 Polyclonal antibody (28334-1-AP)

    WB analysis using 28334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28334-1-AP (HDAC9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using HDAC9 Polyclonal antibody (28334-1-AP)

    IF Staining of SH-SY5Y using 28334-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HDAC9 antibody (28334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inBxPC-3 cells, MDA-MB-231 cells, MDA-MB-468 cells, mouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28334-1-AP targets HDAC9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC9 fusion protein Ag28514 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 9
Calculated molecular weight 1011 aa, 111 kDa
Observed molecular weight57 kDa
GenBank accession numberBC152405
Gene symbol HDAC9
Gene ID (NCBI) 9734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC9, also named as Histone deacetylase 7B, is a 1011 amino acid protein, which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC9 represses MEF2-dependent transcription. HDAC9 is broadly expressed, with highest levels in brain, heart, muscle, and testis. HDAC9 has 11 isoforms produced by alternative splicing. The observed molecular weight of HDAC9 is 57 kDa, which represents alternate isoforms of HDAC9.

Protocols

Product Specific Protocols
WB protocol for HDAC9 antibody 28334-1-APDownload protocol
IF protocol for HDAC9 antibody 28334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols