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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDDC2 Polyclonal antibody

HDDC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16632-1-AP

Synonyms

HCV NS5A-transactivated protein 2, EC:3.1.3.89, CGI-130, CGI 130, C6orf74

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDDC2 Polyclonal antibody (16632-1-AP)

    WB analysis using 16632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16632-1-AP (HDDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using HDDC2 Polyclonal antibody (16632-1-AP)

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HDDC2 Polyclonal antibody (16632-1-AP)

    IHC staining of human brain using 16632-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16632-1-AP (HDDC2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using HDDC2 Polyclonal antibody (16632-1-AP)

    IF Staining of PC-3 using 16632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16632-1-AP (HDDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16632-1-AP targets HDDC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDDC2 fusion protein Ag9971 相同性解析による交差性が予測される生物種
Full Name HD domain containing 2
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC003357
Gene symbol HDDC2
Gene ID (NCBI) 51020
RRIDAB_10597248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

Protocols

Product Specific Protocols
WB protocol for HDDC2 antibody 16632-1-APDownload protocol
IHC protocol for HDDC2 antibody 16632-1-APDownload protocol
IF protocol for HDDC2 antibody 16632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols