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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDGF Monoclonal antibody, PBS Only

HDGF Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A7G3

Cat no : 60064-1-PBS

Synonyms

HDGF, Hepatoma derived growth factor, HMG 1L2, HMG1L2

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using HDGF Monoclonal antibody (60064-1-Ig)

    WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using HDGF Monoclonal antibody (60064-1-Ig)

    WB analysis of rat liver using 60064-1-Ig (same clone as 60064-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HDGF Monoclonal antibody (60064-1-Ig)

    WB analysis of HeLa using 60064-1-Ig (same clone as 60064-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using HDGF Monoclonal antibody (60064-1-Ig)

    WB analysis of A549 using 60064-1-Ig (same clone as 60064-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HDGF Monoclonal antibody (60064-1-Ig)

    WB analysis of HepG2 using 60064-1-Ig (same clone as 60064-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using HDGF Monoclonal antibody (60064-1-Ig)

    WB analysis of L02 using 60064-1-Ig (same clone as 60064-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HDGF Monoclonal antibody (60064-1-Ig)

    IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HDGF Monoclonal antibody (60064-1-Ig)

    IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HDGF Monoclonal antibody (60064-1-Ig)

    IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HDGF Monoclonal antibody (60064-1-Ig)

    IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HDGF Monoclonal antibody (60064-1-Ig)

    IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HDGF Monoclonal antibody (60064-1-Ig)

    IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60064-1-PBS targets HDGF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HDGF fusion protein Ag1893 相同性解析による交差性が予測される生物種
Full Name hepatoma-derived growth factor (high-mobility group protein 1-like)
Calculated molecular weight 26 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC018991
Gene symbol HDGF
Gene ID (NCBI) 3068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)