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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDGFL1 Polyclonal antibody (31923-1-AP)

    WB analysis using 31923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31923-1-AP (HDGFL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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HDGFL1 Polyclonal antibody

HDGFL1 Polyclonal Antibody for WB, ELISA
Cat No. 31923-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HDGF (Hepatoma-Derived Growth Factor) Like , HDGF Like 1 , Hdgfrp1, Hepatoma-derived growth factor-like protein 1, HRP-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31923-1-AP targets HDGFL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDGFL1 fusion protein Ag36944 相同性解析による交差性が予測される生物種
Full Name hepatoma derived growth factor-like 1
Calculated molecular weight27 kDa
Observed molecular weight40 kDa
GenBank accession numberNM_138574
Gene Symbol HDGFL1
Gene ID (NCBI) 154150
RRIDAB_3670147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5TGJ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hepatoma-derived growth factor-like 1 (HDGFL1, also known as HRP-1, PWWP1, and Hdgfrp1) is a member of the hepatoma-derived growth factor (HDGF) family related to tumorigenesis and the development of cancer (PMID: 18823380;  20509080). It is specifically expressed in the testis and activated in the nucleus (PMID: 22217008).

Protocols

Product Specific Protocols
WB protocol for HDGFL1 antibody 31923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols