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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HE4 Monoclonal antibody

HE4 Monoclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5B2D9

Cat no : 66557-1-Ig

Synonyms

HE4, WAP5, WFDC2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HE4 Monoclonal antibody (66557-1-Ig)

    IHC staining of human ovary tumor using 66557-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HE4 Monoclonal antibody (66557-1-Ig)

    IHC staining of human ovary tumor using 66557-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using HE4 Monoclonal antibody (66557-1-Ig)

    IF Staining of SKOV-3 using 66557-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66557-1-Ig targets HE4 in IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HE4 fusion protein Ag5936 相同性解析による交差性が予測される生物種
Full Name WAP four-disulfide core domain 2
Calculated molecular weight 13 kDa
GenBank accession numberBC046106
Gene symbol HE4
Gene ID (NCBI) 10406
RRIDAB_2881918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

Protocols

Product Specific Protocols
IHC protocol for HE4 antibody 66557-1-IgDownload protocol
IF protocol for HE4 antibody 66557-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols