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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEATR3 Polyclonal antibody

HEATR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27335-1-AP

Synonyms

HEAT repeat-containing protein 3, HEAT repeat containing 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEATR3 Polyclonal antibody (27335-1-AP)

    WB analysis using 27335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27335-1-AP (HEATR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HEATR3 Polyclonal antibody (27335-1-AP)

    IHC staining of human ovary cancer using 27335-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27335-1-AP (HEATR3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27335-1-AP targets HEATR3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEATR3 fusion protein Ag26328 相同性解析による交差性が予測される生物種
Full Name HEAT repeat containing 3
Calculated molecular weight 680 aa, 75 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC033077
Gene symbol HEATR3
Gene ID (NCBI) 55027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HEATR3 antibody 27335-1-APDownload protocol
IHC protocol for HEATR3 antibody 27335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols