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  • Featured Product
  • KD/KO Validated

HEBP1 Recombinant antibody

HEBP1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230156B6

Cat no : 82950-1-RR

Synonyms

Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP, HBP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEBP1 Recombinant antibody (82950-1-RR)

    WB analysis using 82950-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82950-1-RR (HEBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using HEBP1 Recombinant antibody (82950-1-RR)

    WB analysis of A549 using 82950-1-RR

    WB result of HEBP1 antibody (82950-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HEBP1 Recombinant antibody (82950-1-RR)

    IHC staining of mouse liver using 82950-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HEBP1 Recombinant antibody (82950-1-RR)

    IHC staining of mouse liver using 82950-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of U2OS cells using HEBP1 Recombinant antibody (82950-1-RR)

    FC experiment of U2OS using 82950-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug HEBP1 Recombinant antibody (82950-1-RR, Clone:230156B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82950-1-RR

    Affinity and Kinetic Characterization of 82950-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82950-1-RR against Human HEBP1 were performed. The affinity constant is 74 nM.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, L02 cells, HepG2 cells, rat liver tissue. mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82950-1-RR targets HEBP1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEBP1 fusion protein Ag10538 相同性解析による交差性が予測される生物種
Full Name heme binding protein 1
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC016277
Gene symbol HEBP1
Gene ID (NCBI) 50865
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HEBP1 antibody 82950-1-RRDownload protocol
IHC protocol for HEBP1 antibody 82950-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols