Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12706-1-AP targets HEBP2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEBP2 fusion protein Ag3387 相同性解析による交差性が予測される生物種 |
| Full Name | heme binding protein 2 |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC008205 |
| Gene Symbol | HEBP2 |
| Gene ID (NCBI) | 23593 |
| RRID | AB_2232981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Z4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HEBP2(Heme-binding protein 2), also named SOUL and C6orf34, can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions (PMID: 17098234). It is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. hEBP2 is essential for the proliferation of human cells and the repression of hEBP2 severely decreases the ability of EBNA1 and EBV-based plasmids to bind mitotic chromosomes (PMID: 15923612).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HEBP2 antibody 12706-1-AP | Download protocol |
| IP protocol for HEBP2 antibody 12706-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol High-dimensional deconstruction of ovarian cancer at single-cell precision reveals HEBP2 that reshape the TIME and drive carboplatin resistance |