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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HECW1 Polyclonal antibody

HECW1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24695-1-AP

Synonyms

HECW1, hNEDL1, KIAA0322, NEDL1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of human urothelial carcinoma using 24695-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of human urothelial carcinoma using 24695-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of mouse cerebellum using 24695-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HECW1 Polyclonal antibody (24695-1-AP)

    IHC staining of mouse cerebellum using 24695-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24695-1-AP (HECW1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman urothelial carcinoma tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24695-1-AP is HECW1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HECW1 fusion protein Ag20264 相同性解析による交差性が予測される生物種
Full Name HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Calculated molecular weight 1606 aa, 180 kDa
GenBank accession numberBC151227
Gene symbol HECW1
Gene ID (NCBI) 23072
RRIDAB_2918085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for HECW1 antibody 24695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols