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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEMGN Polyclonal antibody

HEMGN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17616-1-AP

Synonyms

EDAG, EDAG 1, HEMGN, hemogen, Hemopoietic gene protein, NDR, PRO1620

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HEMGN Polyclonal antibody (17616-1-AP)

    WB analysis of Jurkat using 17616-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using HEMGN Polyclonal antibody (17616-1-AP)

    WB analysis of HL-60 using 17616-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HEMGN Polyclonal antibody (17616-1-AP)

    WB analysis of human testis using 17616-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using HEMGN Polyclonal antibody (17616-1-AP)

    WB analysis of HL-60 using 17616-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using HEMGN Polyclonal antibody (17616-1-AP)

    WB analysis of mouse testis using 17616-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17616-1-AP (HEMGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using HEMGN Polyclonal antibody (17616-1-AP)

    IP experiment of HL-60 using 17616-1-AP

    IP result of anti-HEMGN (IP:17616-1-AP, 4ug; Detection:17616-1-AP 1:500) with HL-60 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using HEMGN Polyclonal antibody (17616-1-AP)

    IHC staining of human testis using 17616-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HEMGN Polyclonal antibody (17616-1-AP)

    IHC staining of human testis using 17616-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17616-1-AP (HEMGN antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, human testis tissue, mouse testis tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17616-1-AP targets HEMGN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEMGN fusion protein Ag11793 相同性解析による交差性が予測される生物種
Full Name hemogen
Calculated molecular weight 483 aa, 55 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC048324
Gene symbol HEMGN
Gene ID (NCBI) 55363
RRIDAB_2117676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HEMGN antibody 17616-1-APDownload protocol
IHC protocol for HEMGN antibody 17616-1-APDownload protocol
IP protocol for HEMGN antibody 17616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biomedicines

Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response

Authors - Yunshu Zhu
View Article