HEPACAM Polyclonal antibody

Name: HEPACAM Polyclonal antibody
Brand: Proteintech
SKU: 18177-1-AP

HEPACAM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18177-1-AP

Synonyms

Protein hepaCAM, Hepatic and glial cell adhesion molecule, glialCAM

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of mouse brain using 18177-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 18177-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 18177-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18177-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18177-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 18177-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18177-1-AP (HEPACAM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of rat brain using 18177-1-AP
IP result of anti-HEPACAM (IP:18177-1-AP, 4ug; Detection:18177-1-AP 1:500) with rat brain tissue lysate 4000ug.
IHC staining of rat brain using 18177-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18177-1-AP (HEPACAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 18177-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18177-1-AP (HEPACAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18177-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18177-1-AP (HEPACAM Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 18177-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18177-1-AP (HEPACAM Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 18177-1-AP
mmunofluorescent analysis of MCF-7 cells, using HEPACAM antibody 18177-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

-/ -

Cat No. 18177-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HepG2 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, SH-SY5Y cells
Positive IP detected in	rat brain tissue
Positive IHC detected in	human liver tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 15 publications below
IHC	See 13 publications below
IF	See 5 publications below
IP	See 1 publications below

Product Information

18177-1-AP targets HEPACAM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HEPACAM fusion protein Ag12870 相同性解析による交差性が予測される生物種
Full Name	hepatocyte cell adhesion molecule
Calculated molecular weight	416 aa, 46 kDa
Observed molecular weight	46-72 kDa
GenBank accession number	BC104831
Gene symbol	HEPACAM
Gene ID (NCBI)	220296
RRID	AB_2279680
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HepaCAM (hepatocyte cell adhesion molecule), also known as GlialCAM, is a single-pass type I membrane glycoprotein of 416 amino acids. It displays a typical structure of immunoglobulin (Ig)-like adhesion molecules including two extracellular Ig-like domains, a transmembrane segment, and a cytoplasmic tail. It has been shown that hepaCAM forms a cis-homodimer on the cell surface, and modulates cell-matrix interaction. It is predominantly expressed in the CNS glial cells. Defects in HEPACAM gene are the cause of megalencephalic leukoencephalopathy with subcortical cysts type 2A (MLC2A). HEPACAM has also been suggested as a tumor suppressor gene.

Protocols

Product Specific Protocols
WB protocol for HEPACAM antibody 18177-1-AP	Download protocol
IHC protocol for HEPACAM antibody 18177-1-AP	Download protocol
IF protocol for HEPACAM antibody 18177-1-AP	Download protocol
IP protocol for HEPACAM antibody 18177-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Hum Mol Genet Megalencephalic leukoencephalopathy with subcortical cysts protein 1 functionally cooperates with the TRPV4 cation channel to activate the response of astrocytes to osmotic stress: dysregulation by pathological mutations. Authors - Lanciotti Angela A View Article
human	WB,IHC	Int J Oncol PMID: 29658567 Authors - Zhongbo Du View Article
human	IHC	Br J Cancer Long noncoding RNA HOTAIR regulates the invasion and metastasis of prostate cancer by targeting hepaCAM. Authors - Ting Li View Article
human	IF	Development Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties. Authors - Robert A Bradley View Article
human	WB,IHC	Cell Signal Interleukin 6 induces cell proliferation of clear cell renal cell carcinoma by suppressing hepaCAM via the STAT3-dependent up-regulation of DNMT1 or DNMT3b. Authors - Zhen Quan View Article
human	WB,IHC	Int J Oncol HepaCAM‑PIK3CA axis regulates the reprogramming of glutamine metabolism to inhibit prostate cancer cell proliferation. Authors - Zhenting He View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEPACAM Polyclonal antibody

HEPACAM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse brain using 18177-1-AP

WB analysis of mouse liver using 18177-1-AP

WB analysis of rat brain using 18177-1-AP

WB analysis of HepG2 using 18177-1-AP

WB analysis of MCF-7 using 18177-1-AP

WB analysis of SH-SY5Y using 18177-1-AP

IP experiment of rat brain using 18177-1-AP

IHC staining of rat brain using 18177-1-AP

IHC staining of rat brain using 18177-1-AP

IHC staining of human liver using 18177-1-AP

IHC staining of human liver using 18177-1-AP

IF Staining of MCF-7 using 18177-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Megalencephalic leukoencephalopathy with subcortical cysts protein 1 functionally cooperates with the TRPV4 cation channel to activate the response of astrocytes to osmotic stress: dysregulation by pathological mutations.

PMID: 29658567

Long noncoding RNA HOTAIR regulates the invasion and metastasis of prostate cancer by targeting hepaCAM.

Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties.

Interleukin 6 induces cell proliferation of clear cell renal cell carcinoma by suppressing hepaCAM via the STAT3-dependent up-regulation of DNMT1 or DNMT3b.

HepaCAM‑PIK3CA axis regulates the reprogramming of glutamine metabolism to inhibit prostate cancer cell proliferation.