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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis of human brain using 13691-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis using 13691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis using 13691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis using 13691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis of human placenta using 13691-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis of HeLa using 13691-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HERC4 Polyclonal antibody (13691-1-AP)

    WB analysis of human testis using 13691-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HERC4 Polyclonal antibody (13691-1-AP)

    IP experiment of HeLa using 13691-1-AP

    IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HERC4 Polyclonal antibody (13691-1-AP)

    IF Staining of HeLa using 13691-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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HERC4 Polyclonal antibody

HERC4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 13691-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human placenta tissue, human testis tissue, mouse testis tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13691-1-AP targets HERC4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HERC4 fusion protein Ag4566 相同性解析による交差性が予測される生物種
Full Name hect domain and RLD 4
Calculated molecular weight 1049 aa, 118 kDa
Observed molecular weight 107 kDa
GenBank accession numberBC039600
Gene Symbol HERC4
Gene ID (NCBI) 26091
RRIDAB_10596480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5GLZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)

Protocols

Product Specific Protocols
WB protocol for HERC4 antibody 13691-1-APDownload protocol
IF protocol for HERC4 antibody 13691-1-APDownload protocol
IP protocol for HERC4 antibody 13691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Elife

SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization.

Authors - Pinxiu Huang
View Article
humanWB,CoIP

Cancer Biother Radiopharm

METTL3 and HERC4: Elevated Expression and Impact on Hepatocellular Carcinoma Progression

Authors - Tao Sun
View Article