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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HERC4 Recombinant antibody (84952-5-RR)

    WB analysis using 84952-5-RR (same clone as 84952-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84952-5-RR (HERC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84952-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84952-5-RR

    Affinity and Kinetic Characterization of 84952-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84952-5-RR against Human HERC4 were performed. The affinity constant is 0.51 nM.

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HERC4 Recombinant antibody, PBS Only

HERC4 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84952-5-PBS
Clone No.242599B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4, EC:2.3.2.26

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84952-5-PBS targets HERC4 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HERC4 fusion protein Ag4566 相同性解析による交差性が予測される生物種
Full Name hect domain and RLD 4
Calculated molecular weight 1049 aa, 118 kDa
Observed molecular weight107 kDa
GenBank accession numberBC039600
Gene Symbol HERC4
Gene ID (NCBI) 26091
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5GLZ8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)