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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HERC5 Polyclonal antibody

HERC5 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22692-1-AP

Synonyms

CEB1, CEBP1, Cyclin E binding protein 1, E3 ISG15 protein ligase HERC5, hect domain and RLD 5, HERC5

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using HERC5 Polyclonal antibody (22692-1-AP)

    IP experiment of HEK-293 using 22692-1-AP

    IP result of anti-HERC5 (IP:22692-1-AP, 4ug; Detection:22692-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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Tested Applications

Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22692-1-AP targets HERC5 in IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HERC5 fusion protein Ag18449 相同性解析による交差性が予測される生物種
Full Name hect domain and RLD 5
Calculated molecular weight 1024 aa, 117 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC140716
Gene symbol HERC5
Gene ID (NCBI) 51191
RRIDAB_2879151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IP protocol for HERC5 antibody 22692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MBio

ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.

Authors - Edmund Osei Kuffour
View Article
humanWB

Int J Mol Med

ISG15 inhibits cancer cell growth and promotes apoptosis.

Authors - Mei-Juan Zhou
View Article
human,mouseWB,CoIP

J Clin Invest

Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation

Authors - Ying Qin
  • KD Validated
View Article
humanWB

Cell Rep

Regulation of STING activity in DNA sensing by ISG15 modification

Authors - Chaohui Lin
View Article
human,mouseCoIP

Cell Rep

ISGylation by HERCs facilitates STING activation

Authors - Ying Qin
View Article
human

Adv Sci (Weinh)

Autophagy Deficiency Induced by SAT1 Potentiates Tumor Progression in Triple-Negative Breast Cancer

Authors - Wenwen Tian
View Article