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- KD/KO Validated
HERP Polyclonal antibody
HERP Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10813-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, Tunicamycin treated HepG2 cells, HeLa cells, LNCaP cells, U2OS cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10813-1-AP targets HERP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HERP fusion protein Ag1084 相同性解析による交差性が予測される生物種 |
| Full Name | homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC008320 |
| Gene symbol | HERPUD1 |
| Gene ID (NCBI) | 9709 |
| RRID | AB_2248317 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HERP antibody 10813-1-AP | Download protocol |
| IHC protocol for HERP antibody 10813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene. | ||
J Alzheimers Dis The Herp protein pathway is not involved in the pro-amyloidogenic effect of hyperhomocysteinemia. | ||
Biol Reprod The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome. | ||
PLoS One C/EBP-beta regulates endoplasmic reticulum stress-triggered cell death in mouse and human models. | ||
Exp Cell Res ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.
| ||
Mol Cell Biochem Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling. |
