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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HERPUD2 Monoclonal antibody

HERPUD2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H10H1

Cat no : 68427-1-Ig

Synonyms

FLJ22313, FLJ31032, HERPUD family member 2, HERPUD2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HERPUD2 Monoclonal antibody (68427-1-Ig)

    WB analysis using 68427-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68427-1-Ig (HERPUD2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HERPUD2 Monoclonal antibody (68427-1-Ig)

    IF Staining of HepG2 using 68427-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HERPUD2 antibody (68427-1-Ig, Clone: 1H10H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inNCI-H1299 cells, L02 clls, HepG2 cells, A549 cells, HT-29 cells, LNCaP cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68427-1-Ig targets HERPUD2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen HERPUD2 fusion protein Ag15007 相同性解析による交差性が予測される生物種
Full Name HERPUD family member 2
Calculated molecular weight 406 aa, 45 kDa
Observed molecular weight35-45 kDa
GenBank accession numberBC005091
Gene symbol HERPUD2
Gene ID (NCBI) 64224
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HERPUD2, also named as Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein, is a 406 amino acid protein, which contains one ubiquitin-like domain. HERPUD2 as a single-pass membrane protein could be involved in the unfolded protein response (UPR) pathway. HERPUD2 may exstis as two isoforms 406aa,45 kDa and 297aa 33 kDa (Genebank:EAW94050).

Protocols

Product Specific Protocols
WB protocol for HERPUD2 antibody 68427-1-IgDownload protocol
IF protocol for HERPUD2 antibody 68427-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols