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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HESX1 Polyclonal antibody

HESX1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17927-1-AP

Synonyms

ANF, hAnf, HESX homeobox 1, HESX1, Homeobox protein ANF, RPX

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse testis tissue using HESX1 Polyclonal antibody (17927-1-AP)

    IHC staining of mouse testis using 17927-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17927-1-AP (HESX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HESX1 Polyclonal antibody (17927-1-AP)

    IHC staining of mouse testis using 17927-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17927-1-AP (HESX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17927-1-AP targets HESX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HESX1 fusion protein Ag12348 相同性解析による交差性が予測される生物種
Full Name HESX homeobox 1
Calculated molecular weight 185 aa, 21 kDa
GenBank accession numberBC093979
Gene symbol HESX1
Gene ID (NCBI) 8820
RRIDAB_2118120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HESX1 (Homeobox gene expressed in stem cells 1) is a homeobox transcriptional repressor that was initially isolated from murine embryonic stem cells (ESCs). Hesx1 is crucial for proper forebrain and pituitary development in both mice and humans, and inactivating mutations in HESX1 underlie septo-optic dysplasia.

Protocols

Product Specific Protocols
IHC protocol for HESX1 antibody 17927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols