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- KD/KO Validated
HEXA Polyclonal antibody
HEXA Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11317-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
11317-1-AP targets HEXA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEXA fusion protein Ag1854 相同性解析による交差性が予測される生物種 |
| Full Name | hexosaminidase A (alpha polypeptide) |
| Calculated molecular weight | 529 aa, 60 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC018927 |
| Gene symbol | HEXA |
| Gene ID (NCBI) | 3073 |
| RRID | AB_2118133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta-hexosaminidase subunit alpha (HEXA) is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HEXA antibody 11317-1-AP | Download protocol |
| IHC protocol for HEXA antibody 11317-1-AP | Download protocol |
| IF protocol for HEXA antibody 11317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation. | ||
Front Oncol Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.
| ||
Proc Natl Acad Sci U S A A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB | ||
Nat Commun Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joshua (Verified Customer) (01-26-2023) | Did not detect expected mature HEXA ~54kDa.
 |
FH Georgina (Verified Customer) (09-15-2022) | Good to have the chance to buy a sample to try out an experiment. The antibody worked.
|
