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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEXA Polyclonal antibody

HEXA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29259-1-AP

Synonyms

HEXA, Hexosaminidase subunit A, TSD

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HEXA Polyclonal antibody (29259-1-AP)

    WB analysis using 29259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29259-1-AP (HEXA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HEXA Polyclonal antibody (29259-1-AP)

    WB analysis using 29259-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29259-1-AP (HEXA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HEXA Polyclonal antibody (29259-1-AP)

    IHC staining of human kidney using 29259-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HEXA Polyclonal antibody (29259-1-AP)

    IHC staining of human kidney using 29259-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using HEXA Polyclonal antibody (29259-1-AP)

    IHC staining of human lung using 29259-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29259-1-AP targets HEXA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEXA fusion protein Ag30808 相同性解析による交差性が予測される生物種
Full Name hexosaminidase A (alpha polypeptide)
Calculated molecular weight 529 aa, 60 kDa
Observed molecular weight 50-54 kDa, 60-67 kDa
GenBank accession numberBC018927
Gene symbol HEXA
Gene ID (NCBI) 3073
RRIDAB_2918259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

Protocols

Product Specific Protocols
WB protocol for HEXA antibody 29259-1-APDownload protocol
IHC protocol for HEXA antibody 29259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Proteome and Glycoproteome Analyses Reveal Regulation of Protein Glycosylation Site-Specific Occupancy and Lysosomal Hydrolase Maturation by N-Glycan-Dependent ER-Quality Control

Authors - Jingru Chen
View Article