Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue, Jurkat cells, HeLa cells, mouse lung tissue, rat kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16229-1-AP targets HEXB in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEXB fusion protein Ag8683 相同性解析による交差性が予測される生物種 |
| Full Name | hexosaminidase B (beta polypeptide) |
| Calculated molecular weight | 556 aa, 63 kDa |
| Observed molecular weight | 63-67 kDa |
| GenBank accession number | BC017378 |
| Gene Symbol | HEXB |
| Gene ID (NCBI) | 3074 |
| RRID | AB_2264075 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07686 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HEXB antibody 16229-1-AP | Download protocol |
| IHC protocol for HEXB antibody 16229-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Neurobiol The Role of Oxidized Cholesterol in Diabetes-Induced Lysosomal Dysfunction in the Brain. | ||
Front Cell Dev Biol Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC. | ||
Front Oncol Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.
| ||
Biol Open Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo. | ||
J Cereb Blood Flow Metab Characteristics of activation of monocyte-derived macrophages versus microglia after mouse experimental intracerebral hemorrhage |