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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEXIM1 Recombinant antibody, PBS Only (Detector)

HEXIM1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242587A2

Cat no : 85028-1-PBS

Synonyms

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HEXIM1 Recombinant antibody (85028-1-RR)

    WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEXIM1 Recombinant antibody (85028-1-RR)

    IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01740-2

    Cytometric bead array standard curve of MP01740-2

    Cytometric bead array standard curve of MP01740-2, HEXIM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85028-3-PBS. Detection antibody: 85028-1-PBS. Standard: Ag8144. Range: 0.156-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85028-1-PBS targets HEXIM1 as part of a matched antibody pair:

MP01740-2: 85028-3-PBS capture and 85028-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEXIM1 fusion protein Ag8144 相同性解析による交差性が予測される生物種
Full Name hexamethylene bis-acetamide inducible 1
Calculated molecular weight 41 kDa
Observed molecular weight65 kDa
GenBank accession numberBC006460
Gene symbol HEXIM1
Gene ID (NCBI) 10614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).