Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of K-562 using 10597-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of 4T1 using 10597-1-AP
4T1 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10597-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10597-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10597-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 10597-1-AP
IP result of anti-HEY2 (IP:10597-1-AP, 5ug; Detection:10597-1-AP 1:500) with mouse heart tissue lysate 7500ug.
IF Staining of HepG2 using 10597-1-AP
Immunofluorescent analysis of HepG2 cells, using HEY2 antibody 10597-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

HEY2 Polyclonal antibody

HEY2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10597-1-AP

Publications(48)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, chIP, ELISA

Synonyms

GRL, GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (48)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells
Positive IP detected in	mouse heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 28 publications below
IHC	See 9 publications below
IF	See 10 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

Product Information

10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HEY2 fusion protein Ag0913 相同性解析による交差性が予測される生物種
Full Name	hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight	36 kDa
Observed molecular weight	33-40 kDa
GenBank accession number	BC007707
Gene Symbol	HEY2
Gene ID (NCBI)	23493
RRID	AB_2118415
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBP5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.

Protocols

Product Specific Protocols
WB protocol for HEY2 antibody 10597-1-AP	Download protocol
IF protocol for HEY2 antibody 10597-1-AP	Download protocol
IP protocol for HEY2 antibody 10597-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Multi-chamber cardioids unravel human heart development and cardiac defects Authors - Clara Schmidt View Article
human	IF	Cell Stem Cell Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Authors - Blair K Gage View Article
mouse	WB	Nat Neurosci Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination. Authors - Lai Man Natalie Wu View Article
human	chIP	Nat Commun Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. Authors - Jared M Churko View Article
human	IF	Nat Commun Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells. Authors - Shunsuke Funakoshi View Article
human	WB	Nat Commun Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. Authors - Haiying Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 10597-1-AP

WB analysis of 4T1 using 10597-1-AP

WB analysis of MCF-7 using 10597-1-AP

WB analysis of mouse heart using 10597-1-AP

WB analysis of K-562 using 10597-1-AP

IP experiment of mouse heart using 10597-1-AP

IF Staining of HepG2 using 10597-1-AP

HEY2 Polyclonal antibody

HEY2 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Multi-chamber cardioids unravel human heart development and cardiac defects

Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.

Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.

Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.

Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.

Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.