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HEY2 Polyclonal antibody
HEY2 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 10597-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 26 publications below |
| IHC | See 9 publications below |
| IF | See 9 publications below |
| CoIP | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HEY2 fusion protein Ag0913 相同性解析による交差性が予測される生物種 |
| Full Name | hairy/enhancer-of-split related with YRPW motif 2 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 33-40 kDa |
| GenBank accession number | BC007707 |
| Gene symbol | HEY2 |
| Gene ID (NCBI) | 23493 |
| RRID | AB_2118415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IF protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IP protocol for HEY2 antibody 10597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. | ||
Nat Neurosci Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination. | ||
Nat Commun Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. | ||
Nat Commun Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells. | ||
Nat Commun Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH CHAO (Verified Customer) (03-31-2022) | Weak band around correct size.
|
FH Tom (Verified Customer) (08-22-2020) | I performed western blot with mouse heart tissue lysate using Hey2 antibody. A specific ~36 kDa Hey2 band was observed.
|
FH Tom (Verified Customer) (08-22-2020) | This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern.
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