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HEY2 Polyclonal antibody

HEY2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 10597-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, chIP, ELISA

GRL, GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inK-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10597-1-AP targets HEY2 in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEY2 fusion protein Ag0913 相同性解析による交差性が予測される生物種
Full Name hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight 36 kDa
Observed molecular weight33-40 kDa
GenBank accession numberBC007707
Gene Symbol HEY2
Gene ID (NCBI) 23493
RRIDAB_2118415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBP5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.

Protocols

Product Specific Protocols
WB protocol for HEY2 antibody 10597-1-APDownload protocol
IF protocol for HEY2 antibody 10597-1-APDownload protocol
IP protocol for HEY2 antibody 10597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Multi-chamber cardioids unravel human heart development and cardiac defects

Authors - Clara Schmidt
humanIF

Cell Stem Cell

Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.

Authors - Blair K Gage
mouseWB

Nat Neurosci

Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.

Authors - Lai Man Natalie Wu
humanchIP

Nat Commun

Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.

Authors - Jared M Churko
humanIF

Nat Commun

Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.

Authors - Shunsuke Funakoshi
humanWB

Nat Commun

Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.

Authors - Haiying Jiang
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