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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEY2 Recombinant antibody

HEY2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240474F2

Cat no : 83501-7-RR

Synonyms

GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1, bHLHb32

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEY2 Recombinant antibody (83501-7-RR)

    IF Staining of HeLa using 83501-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HEY2 Recombinant antibody (83501-7-RR)

    IF Staining of HeLa using 83501-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using HEY2 Recombinant antibody (83501-7-RR)

    FC experiment of HEK-293 using 83501-7-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using HEY2 Recombinant antibody (83501-7-RR)

    FC experiment of HeLa using 83501-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83501-7-RR

    Affinity and Kinetic Characterization of 83501-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83501-7-RR is HEY2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEY2 fusion protein Ag30988 相同性解析による交差性が予測される生物種
Full Name hairy/enhancer-of-split related with YRPW motif 2
Calculated molecular weight 36 kDa
GenBank accession numberBC007707
Gene symbol HEY2
Gene ID (NCBI) 23493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2.

Protocols

Product Specific Protocols
IF protocol for HEY2 antibody 83501-7-RRDownload protocol
FC protocol for HEY2 antibody 83501-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols