• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HGD Polyclonal antibody (16465-1-AP)

    WB analysis using 16465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HGD Polyclonal antibody (16465-1-AP)

    WB analysis using 16465-1-AP

    Rat liver tissues were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HGD Polyclonal antibody (16465-1-AP)

    WB analysis of HeLa using 16465-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using HGD Polyclonal antibody (16465-1-AP)

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HGD Polyclonal antibody (16465-1-AP)

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HGD Polyclonal antibody

HGD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16465-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AKU, HGD, HGO, Homogentisate 1,2 dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16465-1-AP targets HGD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HGD fusion protein Ag9544 相同性解析による交差性が予測される生物種
Full Name homogentisate 1,2-dioxygenase (homogentisate oxidase)
Calculated molecular weight 37 kDa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC020792
Gene Symbol HGD
Gene ID (NCBI) 3081
RRIDAB_2248397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

Protocols

Product Specific Protocols
WB protocol for HGD antibody 16465-1-APDownload protocol
IHC protocol for HGD antibody 16465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols