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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HGD Recombinant antibody (84931-5-RR)

    WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HGD Recombinant antibody (84931-5-RR)

    WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

    rat liver tissue lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84931-5-RR

    Affinity and Kinetic Characterization of 84931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84931-5-RR against Human HGD were performed. The affinity constant is 1.38 nM.

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HGD Recombinant antibody, PBS Only

HGD Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84931-5-PBS
Clone No.242485E11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Homogentisate 1,2-dioxygenase, Homogentisate 1,2 dioxygenase, HGO, EC:1.13.11.5, AKU

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84931-5-PBS targets HGD in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HGD fusion protein Ag9544 相同性解析による交差性が予測される生物種
Full Name homogentisate 1,2-dioxygenase (homogentisate oxidase)
Calculated molecular weight 37 kDa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC020792
Gene Symbol HGD
Gene ID (NCBI) 3081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ93099
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).