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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HGS Monoclonal antibody, PBS Only

HGS Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B10D6

Cat no : 67818-1-PBS

Synonyms

HGS, HRS, Protein pp110, ZFYVE8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HGS Monoclonal antibody (67818-1-Ig)

    WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using HGS Monoclonal antibody (67818-1-Ig)

    WB analysis of PC-12 using 67818-1-Ig (same clone as 67818-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using HGS Monoclonal antibody (67818-1-Ig)

    WB analysis of Neuro-2a using 67818-1-Ig (same clone as 67818-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HGS Monoclonal antibody (67818-1-Ig)

    WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HGS Monoclonal antibody (67818-1-Ig)

    WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HGS Monoclonal antibody (67818-1-Ig)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HGS Monoclonal antibody (67818-1-Ig)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HGS Monoclonal antibody (67818-1-Ig)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HGS Monoclonal antibody (67818-1-Ig)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HGS Monoclonal antibody (67818-1-Ig)

    IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGS antibody (67818-1-Ig, Clone: 3B10D6 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67818-1-PBS targets HGS in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HGS fusion protein Ag28610 相同性解析による交差性が予測される生物種
Full Name hepatocyte growth factor-regulated tyrosine kinase substrate
Calculated molecular weight 86 kDa
Observed molecular weight110 kDa
GenBank accession numberBC003565
Gene symbol HGS
Gene ID (NCBI) 9146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.