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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HHIP Polyclonal antibody

HHIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29466-1-AP

Synonyms

FLJ20992, FLJ90230, hedgehog interacting protein, HHIP, HIP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HHIP Polyclonal antibody (29466-1-AP)

    WB analysis using 29466-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HHIP Polyclonal antibody (29466-1-AP)

    IHC staining of mouse brain using 29466-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using HHIP Polyclonal antibody (29466-1-AP)

    IHC staining of mouse pancreas using 29466-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HHIP Polyclonal antibody (29466-1-AP)

    IHC staining of mouse testis using 29466-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HHIP Polyclonal antibody (29466-1-AP)

    IHC staining of mouse testis using 29466-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using HHIP Polyclonal antibody (29466-1-AP)

    IHC staining of mouse pancreas using 29466-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HHIP Polyclonal antibody (29466-1-AP)

    IF Staining of HeLa using 29466-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HHIP antibody (29466-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse pancreas tissue, mouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29466-1-AP targets HHIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HHIP fusion protein Ag30869 相同性解析による交差性が予測される生物種
Full Name hedgehog interacting protein
Calculated molecular weight 700 aa, 79 kDa
Observed molecular weight85 kDa
GenBank accession numberBC025311
Gene symbol HHIP
Gene ID (NCBI) 64399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. HHIP is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH.

Protocols

Product Specific Protocols
WB protocol for HHIP antibody 29466-1-APDownload protocol
IHC protocol for HHIP antibody 29466-1-APDownload protocol
IF protocol for HHIP antibody 29466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols