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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIBCH Monoclonal antibody, PBS Only

HIBCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7B8

Cat no : 68157-1-PBS

Synonyms

HIB CoA hydrolase, HIBCH, HIBYL COA H

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIBCH Monoclonal antibody (68157-1-Ig)

    WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Monoclonal antibody (68157-1-Ig)

    IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68157-1-PBS targets HIBCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIBCH fusion protein Ag6473 相同性解析による交差性が予測される生物種
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated molecular weight 43 kDa
Observed molecular weight38 kDa
GenBank accession numberBC067822
Gene symbol HIBCH
Gene ID (NCBI) 26275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).