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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIBCH Recombinant antibody, PBS Only (Detector)

HIBCH Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240590A3

Cat no : 83569-1-PBS

Synonyms

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIBCH Recombinant antibody (83569-1-RR)

    WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00560-3

    Cytometric bead array standard curve of MP00560-3

    Cytometric bead array standard curve of MP00560-3, HIBCH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83569-3-PBS. Detection antibody: 83569-1-PBS. Standard: Ag6149. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83569-1-RR

    Affinity and Kinetic Characterization of 83569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83569-1-PBS targets HIBCH as part of a matched antibody pair:

MP00560-3: 83569-3-PBS capture and 83569-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIBCH fusion protein Ag6149 相同性解析による交差性が予測される生物種
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated molecular weight 43 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC067822
Gene symbol HIBCH
Gene ID (NCBI) 26275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).