HIC1 Polyclonal antibody
HIC1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24949-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 24949-1-AP is HIC1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HIC1 fusion protein Ag21849 相同性解析による交差性が予測される生物種 |
| Full Name | hypermethylated in cancer 1 |
| Calculated molecular weight | 714 aa, 75 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC157027 |
| Gene symbol | HIC1 |
| Gene ID (NCBI) | 3090 |
| RRID | AB_2879816 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HIC1 antibody 24949-1-AP | Download protocol |
| IHC protocol for HIC1 antibody 24949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer. | ||
Cancer Cell Int ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression. | ||
Front Immunol Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity |
