- Featured Product
- KD/KO Validated
HIF-1 alpha Monoclonal antibody, PBS Only
HIF-1 alpha Monoclonal Antibody for WB, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
1H3C12
Cat no : 66730-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
66730-1-PBS targets HIF-1 alpha in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HIF-1 alpha fusion protein Ag15198 相同性解析による交差性が予測される生物種 |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Calculated molecular weight | 826 aa, 93 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC012527 |
| Gene symbol | HIF1A |
| Gene ID (NCBI) | 3091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
